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8异前列腺素F2α(8-iso-PGF2a)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0151
  • Price:USD $ 2,800.00
  • Clicks:2336

Product Detail

Product Information

Product Name8-iso-PGF2α(8-isoprostane) ELISA Kit
Alternative Names8-isoprostane; 8-iso-PGF2α

Specifications & Pricing

48T Price1960
96T Price2800
96T*5 Price11900

Assay Parameters

SpeciesGeneral
Assay TypeCompetitive Inhibition
Sensitivity4.47 pg/mL
Detection Range15.63-1000 pg/mL
Sample Typeserum, plasma and other biological fluids
Assay Length2.5h

Research Information

Research AreaEndocrinology;Pulmonology;Hepatology;Hormone metabolism;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-iso-PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-iso-PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-iso-PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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