人喹啉酸磷酸核糖基转移酶(QPRT)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0147
- Price：USD $ 2,800.00
- Clicks：2179
Product Detail
Product Information
| Product Name | Human QPRT(Quinolinate Phosphoribosyltransferase) ELISA Kit |
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| Alternative Names | QPRTase; Nicotinate-Nucleotide Pyrophosphorylase(Carboxylating) |
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Specifications & Pricing
| 48T Price | 1960 |
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| 96T Price | 2800 |
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| 96T*5 Price | 11900 |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 0.048 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | serum, plasma and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Enzyme & Kinase; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human QPRT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human QPRT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human QPRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human QPRT in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | Q15274 |
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Standard Curve
| Standard Curve |  |
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