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大鼠白细胞激活黏附因子(ALCAM)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0084
  • Price:USD $ 2,400.00
  • Clicks:2516

Product Detail

Product Information

Product NameRat ALCAM(Activated Leukocyte Cell Adhesion Molecule) ELISA Kit
Alternative NamesCD166; MEMD; Activated Leucocyte Cell Adhesion Molecule

Specifications & Pricing

48T Price1680
96T Price2400
96T*5 Price10200

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity2.16 ng/mL
Detection Range7.81-500 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCD & Adhesion molecule;Tumor immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ALCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ALCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ALCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ALCAM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDO35112

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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