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马硫代巴比妥酸反应物(TBARS)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0022
  • Price:USD $ 2,800.00
  • Clicks:2395

Product Detail

Product Information

Product NameHorse TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit
Alternative NamesTBARS; Thiobarbituric Acid Reactive Substance

Specifications & Pricing

48T Price1960
96T Price2800
96T*5 Price11900

Assay Parameters

SpeciesHorse
Assay TypeCompetitive Inhibition
Sensitivity3.55 nmol/mL
Detection Range6.25-400 nmol/mL
Sample TypeSerum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length2h

Research Information

Research AreaMetabolic pathway;Hepatology;Hormone metabolism;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse TBARS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse TBARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse TBARS in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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