马硫代巴比妥酸反应物(TBARS)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0022
- Price：Negotiable
- Clicks：2858
Product Detail
Product Information
| Product Name | Horse TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit |
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| Alternative Names | TBARS; Thiobarbituric Acid Reactive Substance |
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Assay Parameters
| Species | Horse |
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| Assay Type | Competitive Inhibition |
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| Sensitivity | 3.55 nmol/mL |
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| Detection Range | 6.25-400 nmol/mL |
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| Sample Type | Serum, plasma, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 2h |
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Research Information
| Research Area | Metabolic pathway;Hepatology;Hormone metabolism; |
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| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse TBARS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse TBARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse TBARS in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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