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Recombinant Human FGFb (157AA)

  • Cat. No.:EPT044
  • Price:USD $ 600.00
  • Clicks:868

Product Detail

Basic Information

Size10ug
Expression HostE.coli
Tag157AA

Physical Properties

Molecular Weight17.4 KDa
Apparent Molecular Weight16 KDa, reducing conditions
FormLyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.
PurityGreater than 95% as determined by reducing SDS-PAGE.

Activity & Endotoxin

Endotoxin LevelLess than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
ActivityMeasured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 1.11 ng/ml.

Storage & Shipping

ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
StorageLyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Shipping ConditionThe product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.

Detailed Description

BackgroundFGF-basic is a members of the Fibroblast Growth Factors (FGFs) family.The family constitutes a large family of proteins involved in many aspects of development including cell proliferation, growth, and differentiation. They act on several cell types to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects, and tissue repair. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4.
For Research Use Only. Not for Diagnostic Procedures.

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