Anti-Human NSUN6 Polyclonal Antibody

Form	Liquid
Host Species	Rabbit
Clonality	Polyclonal
Isotype	IgG
Purification	Purified by antigen affinity column.
Endotoxin Level	Please contact with the lab for this information.
Applications	ELISA, IHC, WB
Species Reactivity	Human
Target	EC:2.1.1.-, NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family member 6, NOPD1, NSUN6, tRNA (cytosine(72)-C(5))-methyltransferase NSUN6
Immunogen	E. coli - derived recombinant Human NSUN6 (Met1-Thr469).
Storage Buffer	0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Product Usage Information	ELISA:1:5000-1:20000;IHC:1:50-1:500;WB:1:500-1:2000
Accession Number	Q8TEA1
Background	tRNA (cytosine(72)-C(5))-methyltransferase NSUN6 is a ~51 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA). In vitro also methylates tRNA(Thr)(AGT). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates. 1. Haag, S. et al. (2015) RNA (New York, N.Y.) 21, 1532-43. PMID: 26160102 2. Long, T. et al. (2016) The Journal of biological chemistry 291, 24293-24303. PMID: 27703015 3. Liu, RJ. et al. (2017) Nucleic acids research 45, 6684-6697. PMID: 28531330
Note	For research use only

For Research Use Only. Not for Diagnostic Procedures.

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