| Form | Liquid |
|---|---|
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Purified by antigen affinity column. |
| Endotoxin Level | Please contact with the lab for this information. |
| Applications | ELISA, IHC, WB |
| Species Reactivity | Measles virus |
| Target | N, NP, Nucleocapsid protein, Nucleoprotein, Protein N |
| Immunogen | E. coli - derived recombinant MeV Nucleoprotein (Thr21-Arg408). |
| Storage Buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
| Product Usage Information | ELISA:1:5000-1:20000;IHC:1:50-1:500;WB:1:500-1:2000 |
| Accession Number | Q77M43 |
| Background | Nucleoprotein is a ~58 kDa protein. Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. The nucleocapsid (NC) has a helical structure with either 12.35 or 11.64 N per turn, approximately 20 nm in diameter, with a hollow central cavity approximately 5 nm in diameter. The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P. 1. Desfosses, A. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 4256-4264. PMID: 30787192 2. Zhou, Y. et al. (2019) Journal of virology 93. PMID: 31375591 |
| Note | For research use only |