| Form | Liquid |
|---|---|
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Purified by antigen affinity column. |
| Endotoxin Level | Please contact with the lab for this information. |
| Applications | ELISA, IHC, WB |
| Species Reactivity | Radiation murine leukemia virus, Friend murine leukemia virus, Xenotropic MuLV-related virus, Murine leukemia virus, AKV murine leukemia virus, Cas-Br-E murine leukemia virus, Moloney murine leukemia |
| Target | CA, Capsid protein p30, EC:2.7.7.-, EC:2.7.7.49, EC:2.7.7.7, EC:3.1.-.-, EC:3.1.26.4, EC:3.4.23.-, Gag-Pol polyprotein, IN, Integrase, MA, Matrix protein p15, NC-pol, Nucleocapsid protein p10-Pol, PR, |
| Immunogen | E. coli - derived recombinant MoMLV Reverse Transcriptase/RT (Thr660-Pro1336). |
| Storage Buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
| Product Usage Information | ELISA:1:5000-1:20000;IHC:1:50-1:500;WB:1:500-1:2000 |
| Accession Number | P03355 |
| Background | Gag-Pol polyprotein (Reverse Transcriptase/RT) is a ~194 kDa protein. Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag-Pol, or to Gag-Pol binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release. |
| Note | For research use only |