Anti-Human Complement C5 Reference Antibody

Form	Liquid
Host Species	Human
Clonality	Monoclonal
Isotype	IgG H-gamma2-4 chimerisotype G2[11aaG4]-kappa
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin Level	< 10 EU/mg
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Species Reactivity	Human
Storage Buffer	0.01 M PBS pH 7.4
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -82°C long term.
Product Usage Information	ELISA: 1:2000-1:20000
Accession Number	P01031
Background	Complement C5 (ComplementC5) is a ~188 kDa protein. Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Activated downstream of classical, alternative, lectin and GZMK complement pathways. 1. Rawal, N. et al. (2003) The Journal of biological chemistry 278, 38476-83. PMID: 12878586 2. Rawal, N. et al. (2008) The Journal of biological chemistry 283, 7853-63. PMID: 18204047 3. Heesterbeek, DA. et al. (2019) The EMBO journal 38. PMID: 30643019 4. DiScipio, RG. et al. (1983) The Journal of biological chemistry 258, 10629-36. PMID: 6554279 5. Donado, CA. et al. (2025) Nature 641, 211-221. PMID: 39914456 6. Lan, F. et al. (2025) Nature 638, 490-498. PMID: 39814882
Note	For research use only.

For Research Use Only. Not for Diagnostic Procedures.

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