大鼠肽基精氨酸脱亚氨酶Ⅳ(PADI4)酶联免疫吸附检测试剂盒
- Cat. No.：ELK11253
- Price：Negotiable
- Clicks：1539
Product Detail
Product Information
| Product Name | Rat PADI4(Peptidyl Arginine Deiminase Type IV) ELISA Kit |
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| Alternative Names | Protein-arginine deiminase type-4;Padi4;PAD-R4;Peptidylarginine deiminase IV;Peptidylarginine deiminase type alpha;Protein-arginine deiminase type IV;Pad4,;Pdi4 |
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Assay Parameters
| Species | Rat |
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| Assay Type | Sandwich |
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| Sensitivity | 6.53 pg/mL |
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| Detection Range | 15.63-1000 pg/mL |
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| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Enzyme & Kinase; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PADI4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PADI4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PADI4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PADI4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | O88807 |
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Standard Curve
| Standard Curve |  |
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