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人谷胱甘肽过氧化酶7(GPX7)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK11238
  • Price:Negotiable
  • Clicks:1999

Product Detail

Product Information

Product NameHuman GPX7(Glutathione Peroxidase 7) ELISA Kit
Alternative NamesGPX6, GPx-7; GSHPx-7,Glutathione peroxidase 7

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.071 ng/mL
Detection Range0.16-10 ng/mL
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;Metabolic pathway;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPX7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPX7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPX7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPX7 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ96SL4

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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