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大鼠转铁蛋白受体(TFR)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK11162
  • Price:Negotiable
  • Clicks:1746

Product Detail

Product Information

Product NameRat TFR(Transferrin Receptor) ELISA Kit
Alternative NamesCD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1, serum form

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.053 µg/mL
Detection Range0.16-10 µg/mL
Sample TypeSerum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TFR in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ99376

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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