大鼠载脂蛋白B(APOB)酶联免疫吸附检测试剂盒

Product Name	Rat APOB(Apolipoprotein B) ELISA Kit
Alternative Names	Apo-B; FLDB; Apo B-100; Apo B-48

Species	Rat
Assay Type	Sandwich
Sensitivity	1.07 µg/mL
Detection Range	3.13-200 µg/mL
Sample Type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length	3.5h

Research Area	Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism;
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APOB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APOB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APOB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APOB in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	Q7TMA5

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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