人纤连蛋白(FN)酶联免疫吸附检测试剂盒

Product Name	Human FN(Fibronectin) ELISA Kit
Alternative Names	FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein

Species	Human
Assay Type	Sandwich
Sensitivity	9.24 ng/mL
Detection Range	31.25-2000 ng/mL
Sample Type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length	3.5h

Research Area	Signal transduction;Infection immunity;
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	P02751

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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