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猫正五聚蛋白3(PTX3)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK11001
  • Price:Negotiable
  • Clicks:1962

Product Detail

Product Information

Product NameCat PTX3(Pentraxin 3, Long) ELISA Kit
Alternative NamesTNFAIP5; TSG-14; Pentaxin-Related Gene,Rapidly Induced By IL-1 Beta; Tumor Necrosis Factor Alpha-Induced Protein 5; Tumor necrosis factor-inducible gene 14 protein; Pentraxin 3; Pentraxin-Related Protein PTX3

Assay Parameters

SpeciesCat
Assay TypeSandwich
Sensitivity0.053 ng/mL
Detection Range0.16-10 ng/mL
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaTumor immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cat PTX3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cat PTX3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cat PTX3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cat PTX3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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