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小鼠白介素1δ(FIL1d)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10912
  • Price:Negotiable
  • Clicks:2029

Product Detail

Product Information

Product NameMouse FIL1d(Interleukin 1 Delta) ELISA Kit
Alternative NamesIL1HY1; IL36RN; IL36Ra; IL1RP3; IL1L1; IL1F5; Interleukin 1 Family, Member 5; Interleukin-1 HY1; IL-1 Related Protein 3; Interleukin 36 Receptor Antagonist

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity5.2 pg/mL
Detection Range15.63-1000 pg/mL
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCytokine;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FIL1d. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FIL1d. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FIL1d, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FIL1d in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ9QYY1

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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