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大鼠去甲肾上腺素(NE)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10884
  • Price:Negotiable
  • Clicks:2176

Product Detail

Product Information

Product NameRat NE(Noradrenaline/Norepinephrine) ELISA Kit
Alternative NamesNA; NAd; Norepinephrine; Norepi

Assay Parameters

SpeciesRat
Assay TypeCompetitive Inhibition
Sensitivity26.2 pg/mL
Detection Range78.13-5000 pg/mL
Sample Typeserum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length2h

Research Information

Research AreaSignal transduction;Infection immunity;Neuro science;Hormone metabolism;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NE in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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