大鼠神经肽FF(NPFF)酶联免疫吸附检测试剂盒

Product Name	Rat NPFF(Neuropeptide FF) ELISA Kit
Alternative Names	FMRFAL; Neuropeptide FF-Amide Peptide Precursor; Pro-FMRFamide-related neuropeptide FF

Species	Rat
Assay Type	Competitive Inhibition
Sensitivity	5.9 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Type	serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length	2h

Research Area	Neuro science;
Test Principle	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NPFF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NPFF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NPFF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	Q9WVA9

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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