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小鼠骨膜蛋白(POSTN)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10803
  • Price:Negotiable
  • Clicks:1666

Product Detail

Product Information

Product NameMouse POSTN(Periostin) ELISA Kit
Alternative NamesOSF-2; PDLPOSTN; PN; Osteoblast Specific Factor 2

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity2.01 ng/mL
Detection Range7.82-500 ng/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse POSTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse POSTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse POSTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse POSTN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ62009

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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