大鼠信号传导转录激活因子6(STAT6)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10787
- Price：Negotiable
- Clicks：2167
Product Detail
Product Information
| Product Name | Rat STAT6(Signal Transducer And Activator Of Transcription 6) ELISA Kit |
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| Alternative Names | IL-4-STAT; STAT6B; STAT6C; Signal Transducer And Activator Of Transcription 6,Interleukin-4 Induced |
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Assay Parameters
| Species | Rat |
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| Assay Type | Sandwich |
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| Sensitivity | 0.057 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | Serum, plasma, tissue homogenates and other biological fluids. |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal transduction;Infection immunity; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat STAT6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat STAT6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat STAT6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat STAT6 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | O70429 |
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Standard Curve
| Standard Curve |  |
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