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小鼠再生胰岛衍生蛋白3α(REG3a)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10785
  • Price:Negotiable
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Product Detail

Product Information

Product NameMouse REG3a(Regenerating Islet Derived Protein 3 Alpha) ELISA Kit
Alternative NamesHIP; INGAP; PAP-H; PAP1; PBCGF; REG-III; REG3; Pancreatitis-Associated Protein; Hepatointestinal pancreatic protein; Human proislet peptide; Pancreatitis-associated protein 1

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.11 ng/mL
Detection Range0.32-20 ng/mL
Sample Typeserum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaInfection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse REG3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse REG3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse REG3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse REG3a in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDO09037

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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