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大鼠血管生成素样蛋白3(ANGPTL3)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10759
  • Price:Negotiable
  • Clicks:1860

Product Detail

Product Information

Product NameRat ANGPTL3(Angiopoietin Like Protein 3) ELISA Kit
Alternative NamesANGPT5; ANG-5; Angiopoietin 5; Angiopoietin-related protein 3

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.64 ng/mL
Detection Range1.56-100 ng/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;Tumor immunity;Hepatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ANGPTL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ANGPTL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ANGPTL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ANGPTL3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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