人高温需求因子A4(HTRA4)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10735
- Price：Negotiable
- Clicks：2592
Product Detail
Product Information
| Product Name | Human HTRA4(High Temperature Requirement Factor A4) ELISA Kit |
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| Alternative Names | HtrA Serine Peptidase 4 |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 1.12 ng/mL |
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| Detection Range | 3.13-200 ng/mL |
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| Sample Type | tissue homogenates, cell lysates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Enzyme & Kinase; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HTRA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HTRA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HTRA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HTRA4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P83105 |
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Standard Curve
| Standard Curve |  |
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