猪高迁移率族蛋白B1(HMGB-1)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10710
- Price：Negotiable
- Clicks：2261
Product Detail
Product Information
| Product Name | Pig HMGB-1(High Mobility Group Protein B1) ELISA Kit |
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| Alternative Names | HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMG1; High Mobility Group Protein 1 |
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Assay Parameters
| Species | Pig |
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| Assay Type | Sandwich |
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| Sensitivity | 0.061 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | Serum, plasma and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Infection immunity; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P12682 |
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Standard Curve
| Standard Curve |  |
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