牛血小板因子4(PF4)酶联免疫吸附检测试剂盒

Product Name	Cattle PF4(Platelet Factor 4) ELISA Kit
Alternative Names	CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact

Species	Cattle
Assay Type	Sandwich
Sensitivity	6.2 ng/mL
Detection Range	15.63-1000 ng/mL
Sample Type	Serum, platelet-poor plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length	3.5h

Research Area	Cytokine;Hematology;
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle PF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle PF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle PF4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	P02777

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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