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马基质金属蛋白酶3(MMP3)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10685
  • Price:Negotiable
  • Clicks:1978

Product Detail

Product Information

Product NameHorse MMP3(Matrix Metalloproteinase 3) ELISA Kit
Alternative NamesSL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1

Assay Parameters

SpeciesHorse
Assay TypeSandwich
Sensitivity0.98 ng/mL
Detection Range3.13-200 ng/mL
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;Tumor immunity;Infection immunity;Cardiovascular biology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse MMP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse MMP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse MMP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse MMP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ28397

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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