猪胰岛素样生长因子结合蛋白1(IGFBP1)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10680
- Price：Negotiable
- Clicks：1787
Product Detail
Product Information
| Product Name | Pig IGFBP1(Insulin Like Growth Factor Binding Protein 1) ELISA Kit |
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| Alternative Names | IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein |
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Assay Parameters
| Species | Pig |
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| Assay Type | Sandwich |
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| Sensitivity | 0.057 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | Serum, plasma and other biological fluids. |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Tumor immunity;Endocrinology;Cardiovascular biology; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IGFBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IGFBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IGFBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig IGFBP1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | Q75ZP3 |
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Standard Curve
| Standard Curve |  |
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