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大鼠补体成分5(C5)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10678
  • Price:Negotiable
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Product Detail

Product Information

Product NameRat C5(Complement Component 5) ELISA Kit
Alternative NamesCPAMD4; C5a anaphylatoxin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.068 µg/mL
Detection Range0.16-10 µg/mL
Sample TypeSerum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;Infection immunity;Immune molecule;Hematology;Autoimmunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat C5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat C5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat C5 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP08650

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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