小鼠α-血红蛋白稳定蛋白(aHSP)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10647
- Price：Negotiable
- Clicks：1855
Product Detail
Product Information
| Product Name | Mouse aHSP(Alpha-Hemoglobin Stabilizing Protein) ELISA Kit |
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| Alternative Names | A-HSP; ERAF; EDRF; Erythroid Associated Factor; Erythroid differentiation-related factor |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.48 ng/mL |
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| Detection Range | 1.57-100 ng/mL |
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| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Metabolic pathway;Infection immunity;Cardiovascular biology; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse aHSP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse aHSP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse aHSP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse aHSP in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | Q9CY02 |
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Standard Curve
| Standard Curve |  |
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