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绵羊组织金属蛋白酶抑制因子1(TIMP1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10645
  • Price:Negotiable
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Product Detail

Product Information

Product NameSheep TIMP1(Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit
Alternative NamesEPO; CLGI; EPA; HCI; Erythroid Potentiating Activity; Collagenase Inhibitor; Metalloproteinase inhibitor 1; Fibroblast collagenase inhibitor

Assay Parameters

SpeciesSheep
Assay TypeSandwich
Sensitivity1.21 ng/mL
Detection Range3.13-200 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaTumor immunity;Infection immunity;Cardiovascular biology;Hepatology;Hormone metabolism;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep TIMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep TIMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep TIMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep TIMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP50122

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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