人胰淀粉酶α2(AMY2)酶联免疫吸附检测试剂盒

Product Name	Human AMY2(Amylase Alpha 2, Pancreatic) ELISA Kit
Alternative Names	AMY2A; AMY2B; PA; 1,4-alpha-D-glucan glucanohydrolase

Species	Human
Assay Type	Sandwich
Sensitivity	1.13 ng/mL
Detection Range	3.13-200 ng/mL
Sample Type	Serum, plasma, tissue homogenates and other biological fluids
Assay Length	3.5h

Research Area	Enzyme & Kinase;Metabolic pathway;Infection immunity;Hepatology;
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AMY2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AMY2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AMY2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AMY2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	P19961

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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