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人凝血因子ⅩⅢB肽(F13B)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10620
  • Price:Negotiable
  • Clicks:1657

Product Detail

Product Information

Product NameHuman F13B(Coagulation Factor XIII B Polypeptide) ELISA Kit
Alternative NamesF13-B; FXIIIB; Fibrin-stabilizing factor B subunit; Protein-glutamine gamma-glutamyltransferase B chain; Transglutaminase B chain

Assay Parameters

SpeciesHuman
Assay TypeCompetitive Inhibition
Sensitivity1.24 ng/mL
Detection Range3.13-200 ng/mL
Sample Typeplasma
Assay Length2h

Research Information

Research AreaSignal transduction;Metabolic pathway;Hematology;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F13B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F13B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F13B in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP05160

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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