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小鼠再生胰岛衍生蛋白1α(REG1a)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10609
  • Price:Negotiable
  • Clicks:1948

Product Detail

Product Information

Product NameMouse REG1a(Regenerating Islet Derived Protein 1 Alpha) ELISA Kit
Alternative NamesREG; PSP; ICRF;  PSPS1; PTP; Islet cells regeneration factor; Islet of Langerhans regenerating protein; Lithostathine 1 Alpha; Pancreatic Stone Protein; Pancreatic Thread Protein

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.049 ng/mL
Detection Range0.16-10 ng/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaInfection immunity;Endocrinology;Hepatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse REG1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse REG1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse REG1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse REG1a in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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