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鸡骨钙素(OC)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10607
  • Price:Negotiable
  • Clicks:2400

Product Detail

Product Information

Product NameChicken OC(Osteocalcin) ELISA Kit
Alternative NamesBGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein

Assay Parameters

SpeciesChicken
Assay TypeSandwich
Sensitivity0.064 ng/mL
Detection Range0.16-10 ng/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken OC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP02822

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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