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小鼠IgE-Fc片段受体Ⅱ(FceRII)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10538
  • Price:Negotiable
  • Clicks:1840

Product Detail

Product Information

Product NameMouse FceRII(Receptor II For The Fc Region Of Immunoglobulin E) ELISA Kit
Alternative NamesCD23; FCER2; BLAST-2; CLEC4J; FCE2; IGEBF; C-type lectin domain family 4 member J; Immunoglobulin E-binding factor; Lymphocyte IgE receptor

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.13 ng/mL
Detection Range0.32-20 ng/mL
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaCD & Adhesion molecule;Infection immunity;Rheumatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FceRII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FceRII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FceRII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FceRII in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP20693

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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