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人补体成分5a(C5a)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK1051
  • Price:Negotiable
  • Clicks:1862

Product Detail

Product Information

Product NameHuman C5a(Complement Component 5a) ELISA Kit
Alternative NamesComplement Component 5a

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity28 pg/mL
Detection Range78.13-5000 pg/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaInfection immunity;Immune molecule;Autoimmunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C5a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C5a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C5a in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP01031

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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