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小鼠类胰蛋白酶(TPS)微量上样酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10506MS
  • Price:Negotiable
  • Clicks:2181

Product Detail

Product Information

Product NameMouse TPS(Tryptase) Microsample ELISA Kit
Alternative NamesTPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.22 ng/mL
Detection Range0.78-50 ng/mL
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;Infection immunity;Rheumatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TPS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TPS in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ02844

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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