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兔肝素辅因子Ⅱ(HCII)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10501
  • Price:Negotiable
  • Clicks:1904

Product Detail

Product Information

Product NameRabbit HCII(Heparin Cofactor II) ELISA Kit
Alternative NamesSERPIND1; HC2; HCF2; HC II; HLS2; LS2; Serpin D1; Serpin Peptidase Inhibitor Clade D Member 1; Protease inhibitor leuserpin-2

Assay Parameters

SpeciesRabbit
Assay TypeSandwich
Sensitivity0.13 ng/mL
Detection Range0.32-20 ng/mL
Sample TypePlasma
Assay Length3.5h

Research Information

Research AreaHematology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit HCII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit HCII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit HCII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit HCII in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP47776

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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