小鼠Ⅲ型胶原(COL3)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10447
- Price：Negotiable
- Clicks：2123
Product Detail
Product Information
| Product Name | Mouse COL3(Collagen Type III) ELISA Kit |
|---|---|
| Alternative Names | Collagen Type 3; Col 3; Collagen Type III; Col III |
Assay Parameters
| Species | Mouse |
|---|---|
| Assay Type | Sandwich |
| Sensitivity | 0.64 ng/mL |
| Detection Range | 1.57-100 ng/mL |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids |
| Assay Length | 3.5h |
Research Information
| Research Area | Metabolic pathway;Cardiovascular biology; |
|---|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse COL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse COL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse COL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse COL3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
| Standard Curve |  |
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