| Product Name | Human AP36(Apelin 36) ELISA Kit |
|---|---|
| Alternative Names | APLN36 |
| Species | Human |
|---|---|
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.071 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Length | 2h |
| Research Area | Metabolic pathway;Endocrinology;Gastroenterology; |
|---|---|
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP36. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP36. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP36 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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