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小鼠克拉拉细胞蛋白16(CC16)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10307
  • Price:Negotiable
  • Clicks:2016

Product Detail

Product Information

Product NameMouse CC16(Clara Cell Protein 16) ELISA Kit
Alternative NamesSCGB1A1; CC10; CCSP; UGB; Uteroglobin; Urinary Protein 1; Clara Cell Secretory Protein; Secretoglobin Family 1A Member 1; Clara-Cell Specific 10-kD Protein

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity1.06 ng/mL
Detection Range3.13-200 ng/mL
Sample Typeserum, plasma, lung lavage fluid, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaInfection immunity;Cardiovascular biology;Pulmonology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CC16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CC16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CC16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CC16 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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