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大鼠凝血因子Ⅸ(F9)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10295
  • Price:Negotiable
  • Clicks:1595

Product Detail

Product Information

Product NameRat F9(Coagulation Factor IX) ELISA Kit
Alternative NamesHEMB; FIX; GLA Domain; PTC; Anti Hemophilic Factor B; Christmas Factor; Plasma Thromboplastic Component; Christmas Disease; Hemophilia B

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.67 ng/mL
Detection Range1.57-100 ng/mL
Sample TypePlasma.
Assay Length3.5h

Research Information

Research AreaSignal transduction;Hematology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat F9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat F9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F9 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP16296

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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