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大鼠组织因子通道抑制因子(TFPI)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10281
  • Price:Negotiable
  • Clicks:2830

Product Detail

Product Information

Product NameRat TFPI(Tissue Factor Pathway Inhibitor) ELISA Kit
Alternative NamesTFI; EPI; LACI; TFPI1; Lipoprotein-Associated Coagulation Inhibitor; Extrinsic pathway inhibitor

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.61 ng/mL
Detection Range1.57-100 ng/mL
Sample Typeplasma, urine, tissue homogenates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;Hematology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TFPI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TFPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TFPI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TFPI in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ02445

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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