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小鼠水通道蛋白4(AQP4)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10280
  • Price:Negotiable
  • Clicks:2138

Product Detail

Product Information

Product NameMouse AQP4(Aquaporin 4) ELISA Kit
Alternative NamesHMIWC2; MIWC; WCH4; Mercurial-insensitive water channel

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.43 ng/mL
Detection Range1.57-100 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;Metabolic pathway;Infection immunity;Neuro science;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AQP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AQP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AQP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AQP4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP55088

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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