Your Position:Home>Products>ELISA Kits>人碳酸酐酶Ⅰ(CA1)酶联免疫吸附检测试剂盒

人碳酸酐酶Ⅰ(CA1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10271
  • Price:Negotiable
  • Clicks:2366

Product Detail

Product Information

Product NameHuman CA1(Carbonic Anhydrase I) ELISA Kit
Alternative NamesCA-I; CAI; Car1; CAB; Carbonic anhydrase B; Carbonate dehydratase I

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.053 ng/mL
Detection Range0.16-10 ng/mL
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;Bone metabolism;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP00915

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

Related Products:
Total 14560 records,Divided into 728 pages,The 2 pages | First Prev Next Last
Live Chat
  • Tel:
  • WeChat ID: 15898894496
  • Add Message
  • 9:00 AM – 6:00 PM