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人肺部激活调节趋化因子(PARC)微量上样酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10202MS
  • Price:Negotiable
  • Clicks:1923

Product Detail

Product Information

Product NameHuman PARC(Pulmonary Activation Regulated Chemokine) Microsample ELISA Kit
Alternative NamesCCL18; AMAC1; SCYA18; DCCK1; MIP-4; CKb7; Alternative Macrophage Activation Associated CC Chemokine 1; Chemokine C-C-Motif Ligand 18; Macrophage inflammatory protein 4

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity1.13 ng/mL
Detection Range3.13-200 ng/mL
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCytokine;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PARC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PARC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PARC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PARC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP55774

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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