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斑马鱼胰岛素(INS)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10167
  • Price:Negotiable
  • Clicks:2431

Product Detail

Product Information

Product NameZebrafish INS(Insulin) ELISA Kit
Alternative NamesInsulin

Assay Parameters

SpeciesZebrafish
Assay TypeSandwich
Sensitivity0.045 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;Endocrinology;Hormone metabolism;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish INS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish INS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish INS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish INS in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDO73727

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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