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一步法小鼠新喋呤(NPT)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK100ES
  • Price:Negotiable
  • Clicks:2099

Product Detail

Product Information

Product NameEasyStep Mouse NPT(Neopterin) ELISA Kit
Alternative NamesNeopterin

Assay Parameters

SpeciesMouse
Assay TypeCompetitive Inhibition
Sensitivity0.4 nmol/L
Detection Range0.469-30 nmol/L
Sample TypeSerum, plasma
Assay Length1.5h

Research Information

Research AreaCytokine;Infection immunity;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NPT antigen, and the Mouse NPT standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse NPT to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse NPT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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