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斑马鱼溶菌酶(LZM)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10068
  • Price:Negotiable
  • Clicks:2018

Product Detail

Product Information

Product NameZebrafish LZM(Lysozyme) ELISA Kit
Alternative NamesLYZ; Renal Amyloidosis; N-Acetylmuramide Glycanhydrolase; Muramidase; 1,4-beta-N-acetylmuramidase C

Assay Parameters

SpeciesZebrafish
Assay TypeSandwich
Sensitivity1.56 ng/mL
Detection Range3.13-200 ng/mL
Sample TypeSerum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish LZM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish LZM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish LZM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish LZM in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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